Identification and Characterization of the LysR - type Transcriptional 1 Regulator HsdR for Steroid - inducible Expression of the 3 α - Hydroxysteroid 2 Dehydrogenase / Carbonyl Reductase Gene in Comamonas testosteroni
نویسندگان
چکیده
Wenjie Gong, Guangming Xiong, Edmund Maser* 5 6 Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School, Schleswig7 Holstein, Campus Kiel, Brunswiker Strasse 10,D-24105 Kiel, Germany 8 9 *Corresponding author: Prof. Edmund Maser, Tel.: +49-431-597-3540; fax: +49-431-597-3558; 10 E-mail address: [email protected] (E. Maser) 11 12 Running Title: HsdR positively regultates the 3α-HSD/CR expression. 13 14 15 16 17 18 19 20 21 22 23 24 Copyright © 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.06872-11 AEM Accepts, published online ahead of print on 9 December 2011
منابع مشابه
Molecular Cloning, Overexpression, and Characterization of Steroid-inducible 3a-Hydroxysteroid Dehydrogenase/Carbonyl Reductase from Comamonas testosteroni A NOVEL MEMBER OF THE SHORT-CHAIN DEHYDROGENASE/REDUCTASE SUPERFAMILY*
3a-Hydroxysteroid dehydrogenase/carbonyl reductase (3a-HSD/CR) from Comamonas testosteroni, a bacterium that is able to grow on steroids as the sole carbon source, catalyzes the oxidoreduction at position 3 of a variety of C19–27 steroids and the carbonyl reduction of a variety of nonsteroidal aldehydes and ketones. The gene of this steroid-inducible 3a-HSD/CR was cloned by screening a C. testo...
متن کاملMolecular Cloning, Overexpression, and Characterization of Steroid-inducible 3a-Hydroxysteroid Dehydrogenase/Carbonyl Reductase from Comamonas testosteroni
3a-Hydroxysteroid dehydrogenase/carbonyl reductase (3a-HSD/CR) from Comamonas testosteroni, a bacterium that is able to grow on steroids as the sole carbon source, catalyzes the oxidoreduction at position 3 of a variety of C19–27 steroids and the carbonyl reduction of a variety of nonsteroidal aldehydes and ketones. The gene of this steroid-inducible 3a-HSD/CR was cloned by screening a C. testo...
متن کاملTeiR, a LuxR-type transcription factor required for testosterone degradation in Comamonas testosteroni.
We have identified a new steroid-inducible gene (designated teiR [testosterone-inducible regulator]) in Comamonas testosteroni that is required for testosterone degradation. Nucleotide sequence analysis of teiR predicts a 391-amino-acid protein which shows homology between residues 327 and 380 (C-terminal domain) to the LuxR helix-turn-helix DNA binding domain and between residues 192 and 227 t...
متن کاملTestosterone-inducible regulator is a kinase that drives steroid sensing and metabolism in Comamonas testosteroni.
The mechanism of gene regulation by steroids in bacteria is still a mystery. We use steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) as a reporter system to study steroid signaling in Comamonas testosteroni. In previous investigations we cloned and characterized the 3alpha-HSD/CR-encoding gene, hsdA. In addition, we identified two negative regulator genes...
متن کاملSteroid degradation and two steroid-inducible enzymes in the marine bacterium H5.
Natural and synthetic steroid hormones excreted into the environment are potentially threatening the population dynamics of all kinds of animals and public health. We have previously isolated a steroid degrading bacterial strain (H5) from the Baltic Sea, at Kiel, Germany. 16S-rRNA analysis showed that bacterial strain H5 belongs to the genus Vibrio, family Vibrionaceae and class Gamma-Proteobac...
متن کامل